Fig. 2: Neuronal activity in brain regions that are associated with anxiety behavior is altered in the PAE mice. | Translational Psychiatry

Fig. 2: Neuronal activity in brain regions that are associated with anxiety behavior is altered in the PAE mice.

From: Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders

Fig. 2

A, E, K c-Fos was detected by immunohistochemistry, and the number of c-Fos+ cells was quantified in the brain regions highlighted in the schematics. B In the ACC, a significant interaction between the exposure and behavior test was observed, F(1,24) = 8.257, p = 0.0084 by two-way ANOVA. After the EPM test, the number of c-Fos+ cells was reduced in PAE (*adj. p = 0.0137 by simple main effects test). C, D No difference was found in the M1 and CP by two-way ANOVA. F There were significant differences in the number of c-Fos+ cells between different treatments and between naïve and post EPM tests in the BLA region. F(1,24) = 9.428, p = 0.0052 and F(1,24) = 5.914, p = 0.0229, respectively by two-way ANOVA. No interaction between these two factors was observed. There was an increase in c-Fos+ cells in PAE mice after the EPM test (*adj. p = 0.0497, Tukey’s post hoc test). G–J No significant changes in the PVH, dCA1, dCA3, or DG regions were observed. L No statistical significance in the vCA1 region was observed. M In the vCA3 region, there was a significant difference in the number of c-Fos+ cells between indicated exposure types. F(1,24) = 9.305, p = 0.0055 by two-way ANOVA. No interaction between the two measures was detected. Tukey’s post hoc test revealed a significant increase in c-Fos+ cells in the naïve PAE mice (*adj. p = 0.0367) compared to the naïve control mice. B–D, F–J, L, M Data are expressed as fold change as compared to naïve control. Graphs represent mean fold change ± SEM, and each dot represents an individual animal. n = 7 per group except for PVH after the EPM test (n = 5 per group). N, O Representative images of the c-Fos immunohistochemistry in the ACC and the BLA, respectively, with higher magnification images in inserts. Scale bars = 200 µm. ACC anterior cingulate cortex, M1 primary motor cortex, CP caudoputamen, BLA basolateral amygdala, PVH paraventricular hypothalamus, dCA1 dorsal CA1 of the hippocampus, dCA3 dorsal CA3 of the hippocampus, DG dentate gyrus, vCA1 ventral CA1 of the hippocampus, vCA3 ventral CA3 of the hippocampus.

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