Fig. 4: Effects of NFAT inhibitors on depression-like phenotype, spleen weight and pro-inflammatory cytokines after LPS injection. | Translational Psychiatry

Fig. 4: Effects of NFAT inhibitors on depression-like phenotype, spleen weight and pro-inflammatory cytokines after LPS injection.

From: Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine

Fig. 4

A Treatment schedule. Mice were i.p. injected with LPS (1.0 mg/kg) or saline (10 ml/kg). NFAT inhibitor (10 μM, 2 μl) or saline (2 μl) was administered i.c.v. to mice 60 min prior to LPS injection. Locomotion test and forced swimming test (FST) were performed 23 and 24 h after the injection of saline or LPS, respectively. Blood and spleens were collected after behavioral tests. B Body weight change (one way ANOVA: F2,29 = 27.72, P < 0.0001). C Locomotion test (one way ANOVA: F2,29 = 2.589, P = 0.092). D FST (one way ANOVA: F2,29 = 6.486, P = 0.005). E Spleen weight (one way ANOVA: F2,28 = 26.41, P < 0.0001). F Plasma levels of IL-6 (one way ANOVA: F2,28 = 28.67, P < 0.0001). G Plasma levels of TNF-α (one way ANOVA: F2,28 = 5.013, P = 0.014). H Treatment schedule. Mice were i.p. injected with LPS (1.0 mg/kg) or saline (10 ml/kg). Cyclosporin A (CysA: 40 mg/kg) or vehicle (10% DMSO, 10 ml/kg) was i.p. injected to mice 60 min prior to LPS injection. Locomotion test and FST were performed 23 and 24 h after the injection of saline or LPS, respectively. I Body weight change (one way ANOVA: F2,27 = 90.99, P < 0.0001). J FST (one way ANOVA: F2,27 = 10.86, P = 0.0003). The data represent mean ± S.E.M. (n = 10–12). *P < 0.05, **P < 0.01, ***P < 0.001. N.S., not significant.

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