Fig. 5: Effects of NFATc4 knockdown on depression-like phenotype, spleen weight and inflammatory cytokines after LPS injection. | Translational Psychiatry

Fig. 5: Effects of NFATc4 knockdown on depression-like phenotype, spleen weight and inflammatory cytokines after LPS injection.

From: Nuclear factor of activated T cells 4 in the prefrontal cortex is required for prophylactic actions of (R)-ketamine

Fig. 5

A Treatment schedule. AAV-U6-shNfatc4-CAGGS-EmGFP or AAV-CAGGS-EGFP was injected bilaterally to mPFC 21 days prior to saline (10 ml/kg) or LPS (1.0 mg/kg) injection. Locomotion test and FST were performed 23 and 24 h after the injection of saline or LPS, respectively. Blood, spleen and mPFC were collected after behavioral tests. B Schematic of AAV-mediated Nfatc4 down expression in the mPFC. The diagram shows the AAV constructs and stereotaxic injection of AAV into the mPFC. C The protein expression of phosphorylated NFATc4 (p-NFATc4) in the mPFC (one way ANOVA: F2,26 = 5.440, P = 0.011). D The protein expression of total NFATc4 in the mPFC (one way ANOVA: F2,26 = 6.673, P = 0.005). E Body weight change (one way ANOVA: F2,26 = 36.65, P < 0.0001). F FST (one way ANOVA: F2,26 = 8.409, P = 0.002). G Spleen weight (one way ANOVA: F2,26 = 23.94, P < 0.0001). H Plasma levels of IL-6 (one way ANOVA: F2,26 = 8.580, P = 0.001). I Plasma levels of TNF-α (one way ANOVA: F2,26 = 79.13, P < 0.0001). The data represent mean ± S.E.M. (n = 9–11). *P < 0.05, **P < 0.01, ***P < 0.001. N.S., not significant.

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