Fig. 4: GABAergic signaling regulates acute contextual fear response.
From: TrkA-cholinergic signaling modulates fear encoding and extinction learning in PTSD-like behavior
A, D Schematic representation of the protocols used to test GABAergic and cholinergic signaling role in contextual fear response. B, C Quantification of the freezing response of WT mice treated with donepezil (DON, B) or midazolam (Mid, C) 45 min before (PreFC DON or PreFC Mid) or immediately after (PostFC DON or PostFC Mid) contextual fear conditioning showing that augmentation of GABAergic signaling with midazolam reduces fear response when injected either before or immediately after contextual fear conditioning. *p < 0.05, non-parametric ANOVA followed by Dunn’s multiple comparison test, n = 8/group). E The GABA antagonist picrotoxin injected (0.5 mg/kg intraperitoneal) 45 min before fear conditioning restores the fear response of TrkA-KFG mice to WT control levels. N = 8–10/group. Note that picrotoxin, per se, does not alter the fear response in WT animals. *p < 0.05, Two-way ANOVA followed by Tukey Multiple comparison test.
