Fig. 3: Effects of Torin1 and LY2584702 in primary neuronal cells. | Translational Psychiatry

Fig. 3: Effects of Torin1 and LY2584702 in primary neuronal cells.

From: Differential expression of gene co-expression networks related to the mTOR signaling pathway in bipolar disorder

Fig. 3

Neuronal cells were treated with Torin1 (A) and LY2584702 (B) for 24 h at 250 nM concentration. Cell lysates were analysed by SDS-PAGE and Western blot analyses with each of the primary antibodies. Western blot analyses revealed the levels of phospho-Ser473-Akt, phospho-Ser2448-mTORC1, phospho-Thr389-S6K, phospho-Ser240/244-S6, PSD-95, GluA1, LC3B-II, and Beclin 1.Representative images and quantitative analyses normalized to the total levels for each protein or α-tubulin are shown. The original images for crude blot are shown in Supplementary Fig. 4 (Torin1) and in Supplementary Fig. 6 (LY2584702). Values (n = 4–6) are shown as the mean ± SEM expressed as a percentage of the control cells (no drug) values. *p < 0.05, **p < 0.01, unpaired Student t test.

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