Fig. 2: NMDAR currents in human iPSC-derived neurons. | Translational Psychiatry

Fig. 2: NMDAR currents in human iPSC-derived neurons.

From: Targeting NMDA receptors in neuropsychiatric disorders by drug screening on human neurons derived from pluripotent stem cells

Fig. 2

A Traces displaying NMDA-evoked currents in 3- and 8-week-old human NGN2 iPSC-derived neurons, which were differentiated with a rapid single-step NGN2 induction. B A representative trace shows NMDA-evoked currents in a human iPSC-derived neuron, which was differentiated through an NPC stage in 8-week-old culture, and the effects of Ro 25-6981 (1 µM), a selective antagonist of GluN2B-containing NMDARs and NVP-AAM077 (0.4 µM), an antagonist of GluN2A-containing NMDARs. C Left, Traces showing mEPSCs recorded at the membrane potentials of −40 mV (bottom) and +40 mV (upper) in presence of extracellular Mg2+ at 2 mM in a human iPSC-derived neuron, which was differentiated through an NPC stage in about 4-month-old culture. Middle, Averaged mEPSCs at −40 mV and +40 mV in this cell. Right, A plot depicting the current-voltage relationship of AMPA-mEPSCs and NMDAR-mEPSCs in this cell.

Back to article page