Fig. 1: Identification of major cell types within the CeA.

A UMAP plot of 58,640 CeA single-nuclei colored by cluster. Louvain clustering analysis (resolution = 0.1) identified 18 transcriptionally defined clusters. B UMAP plots showing the expression levels of select neuronal marker genes. Distinct populations of cells express Gad1, a marker of GABAergic inhibitory neurons, and Slc17a7 (VGlut2), a marker of glutamatergic excitatory neurons. These data illustrate that one cluster is composed of primarily excitatory neurons, while multiple clusters are identified which represent distinct inhibitory cell classes of the CeA. C Major cell-type marker gene expression in CeA clusters. The identity of each cluster (0–17) was identified based on the expression of known marker genes for the major cell classes within the CeA. The size of each dot indicates the percentage of cells that contained at least one read of each marker gene; the color depth represents the log-scaled average expression, where the darkest color is the highest expressing cluster. The 24 marker genes that are displayed were identified based on previous studies of the central amygdala [26,27,28,29]. These genes were used to identify each cluster in the dataset. Two clusters (4 and 17) were not readily identifiable based on marker gene expression. D UMAP plot colored by the major cell types identified. Cells were classified as one of nine major cell types. Unidentified clusters comprised two additional groups, for a total of 11 types of cells in the data.