Fig. 1: Generation of the SLC39A8-p.393T knock-in mouse model.
From: The schizophrenia-associated missense variant rs13107325 regulates dendritic spine density
CRISPR-Cas9-mediated genome editing was used to construct the SLC39A8-p.393T knock-in mouse model. Human and mouse SLC39A8 gene showed high-degree sequence and structure similarities. The missense SNP rs13107325 is located in the eighth exon of SLC39A8 and encodes p.Ala391T amino acid, which corresponds to mouse SLC39A8-p.393. Sanger sequencing showed that SLC39A8-p.393T knock-in mouse model was successfully generated. The position of SLC39A8-p.393T (corresponds to human rs13107325) was marked by blue dashed box. WT: wild-type mice (SLC39A8-p.393A); MT: knock-in mice (SLC39A8-p.393T).
