Fig. 1: Expression of NRSF/REST and its target-genes in reduced EHMT1 activity and KS patient iPSC. | Translational Psychiatry

Fig. 1: Expression of NRSF/REST and its target-genes in reduced EHMT1 activity and KS patient iPSC.

From: NRSF/REST lies at the intersection between epigenetic regulation, miRNA-mediated gene control and neurodevelopmental pathways associated with Intellectual disability (ID) and Schizophrenia

Fig. 1

A Gene analysis using qRT-PCR of Ehmt1−/+ mESC-derived NPCs compared to wild type cells. Expression of NRSF/REST mRNA was decreased while neuronal genes regulated by REST were elevated in Ehmt1−/+ cells. Mean fold change over wild type NPCs, n ≥ 3 independent experiments, log10 scale axis. B–D Western blot analysis of NRSF/REST and H3K9me2 protein in pluripotent cells: (B) mESC following treatment with range of UNC0638 concentrations for 48 h; (C) KS patient (2 patients; KS1 and KS2) and EHMT1−/+ iPSC (denoted as E) compared to the isogenic control hiPSC; (D) Nonpatient control iPSC treatment with range of UNC0638 concentrations for 72 h. Plotted as Western band intensity normalized to GAPDH, n ≥ 3. E, F Expression of REST-target genes NRXN3, Calbindin (CALB1) and L1Cam in pluripotent hiPSC, measured by qRT-PCR: (E) control hiPSC treated with 250 nM UNC0636 for 72 h (E), and (F) pluripotent KS patient iPSC. Mean Fold change greater than untreated control hiPSC (log10 axis), n ≥ 3 independent experiments. Data were presented as Mean±SEM and analysed by student’s t-test or One-way ANOVA with post hoc comparisons using Dunnett’s multiple comparisons test comparing to control samples. *P < 0.05, **P < 0.01, ***P < 0.001.

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