Fig. 3: Reduced EHMT1 activity elevates REST-target gene expression during neuronal development. | Translational Psychiatry

Fig. 3: Reduced EHMT1 activity elevates REST-target gene expression during neuronal development.

From: NRSF/REST lies at the intersection between epigenetic regulation, miRNA-mediated gene control and neurodevelopmental pathways associated with Intellectual disability (ID) and Schizophrenia

Fig. 3

A Western blot analysis of NRSF/REST protein expression in hiPSCs-derived neurons at Day 20 (NPC stage) and maturing neurons at Days 35, 40 and 45 of differentiation. NRSF/REST protein was decreased in EHMT1−/+-derived neurons. Quantification of Western blot analysis was performed by normalization to GAPDH. A representative image from at least three independent experiments is shown, with all vales shown on the graph above. B Time-course qRT-PCR analysis at Days 15 and 20 of differentiation to examine changes in the expression of lineage-specific REST-target genes NGN2 and MASH1 in EHMT1−/+-derived neurons compared to their isogenic control hiPSCs. Relative changes is expressed as mean fold change over pluripotent cells, n ≥ 3 independent experiments. C Relative mRNA abundance measured by qRT-PCR of MASH1 in wild type hiPSCs treated with UNC0638. Induction of RESTΔUTR with doxycycline (DOX) suppresses the UNC0638-induced MASH1gene expression. Data were presented as Mean±SEM and analysed by student’s t-test or One-way ANOVA with post hoc comparisons using Dunnett’s multiple comparisons test comparing to control samples.

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