Fig. 4: Reduced EHMT1 activity results in accelerated neuronal development. | Translational Psychiatry

Fig. 4: Reduced EHMT1 activity results in accelerated neuronal development.

From: NRSF/REST lies at the intersection between epigenetic regulation, miRNA-mediated gene control and neurodevelopmental pathways associated with Intellectual disability (ID) and Schizophrenia

Fig. 4

qRT-PCR analysis at days 10 and 20 of differentiation to examine the expression of the neural progenitor markers, (A) Nestin and (B) PAX6 in EHMT1−/+-derived NPC relative to expression in the isogenic control. C Cell staining of two KS patient iPSC developed to NPC (Day 20) stage and stained for Nestin and PAX6 protein. Scale Bar, 50 μm. Graphs show quantitation of protein expression as: stained cell area (Nestin); stained (PAX6+) and mean fluorescence intensity, with DAPI nuclear counterstain. D qRT-PCR analysis of NCAM at days 20 and 25 of early stage differentiated neurons in EHMT1−/+-derived relative to expression in the isogenic control. E qRT-PCR analysis at days 15, 20 and 40 of differentiation to examine changes in the expression of the neuronal marker MAP2 in EHMT1−/+-derived neurons relative to their expression in the isogenic control neurons. F UNC0638 (250 nM) induced expression of MAP2 in differentiating wild-type hiPSC-derived neurons, monitored by qRT-PCR analysis over a time course of 5, 10, 30 and 40 days of treatment. G Relative mRNA abundance measured by qRT-PCR of Nestin and MAP2 in wild-type hiPSC treated with UNC0638. Induction of RESTΔUTR with doxycycline (DOX) suppresses the UNC0638-induced MASH1 gene expression. n ≥ 3 independent experiments. Data were presented as Mean±SEM and analysed by One-way ANOVA with post hoc comparisons using Dunnett’s multiple comparisons test comparing to control samples.

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