Fig. 2: The influence of chronic morphine on the signaling molecules of CREB, ERK, and p90RSK in rat mPFC. | Translational Psychiatry

Fig. 2: The influence of chronic morphine on the signaling molecules of CREB, ERK, and p90RSK in rat mPFC.

From: Two kinds of transcription factors mediate chronic morphine-induced decrease in miR-105 in medial prefrontal cortex of rats

Fig. 2

a The influence of naloxone on the miR-105 expression in primary cultured mPFC neuron treated by morphine (n = 3 in each group, unpaired two-tailed Student’s t-test, ***P < 0.0001). b The expressions of p-CREB to total CREB in rat mPFC (left panel) and primary cultured mPFC neurons (right panel) (n = 3 in each group, unpaired two-tailed Student’s t-test, *P < 0.05, **P < 0.01). c The expressions of p-ERK/ERK and pp90RSK/p90RSK in rat mPFC and primary cultured mPFC neurons (n = 3 in each group, two-tailed Student’s t-test, *P < 0.05). d The expression of miR-105 in primary cultured mPFC neurons of DMSO group, DMSO + morphine group; U0126 + morphine group and LJI308 + morphine group (n = 3 in each group, one-way ANOVA, F(3,8) = 37.40, P < 0.0001. Bonferroni post hoc: **P = 0.0005, DMSO vs DMSO + Morphine, ##P = 0.006 and 0.000, Morphine+U0126 and Morphine+LJI308 vs Morphine group, respectively). Data are presented as mean ± SEM.

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