Fig. 3: Nrf2 and TREM2 protein levels and the microglial arginase 1+ phenotype in the mPFC are involved in depression-like behavior in CSDS mice. | Translational Psychiatry

Fig. 3: Nrf2 and TREM2 protein levels and the microglial arginase 1+ phenotype in the mPFC are involved in depression-like behavior in CSDS mice.

From: Nrf2 regulates the arginase 1+ microglia phenotype through the initiation of TREM2 transcription, ameliorating depression-like behavior in mice

Fig. 3

A Schedule of behavior tests and treatment. B The social interaction test for no-target and target time (mean ± SEM, n = 8 or 9 per group, one-way ANOVA, **p < 0.01, ***p < 0.001). C The sucrose preference test (mean ± SEM, n = 8 or 9 per group, one-way ANOVA, **p < 0.01). D–F Representative images and quantification of TREM2, IBA1, and arginase 1 staining in the mPFC. Scale bar = 50 μm (mean ± SEM, n = 5 per group, one-way ANOVA, ***p < 0.001). G qPCR assay for IL-4 and IL-10 in the mPFC. The data are shown as the mean ± SEM (n = 5 or 6). *P < 0.05 and ***P < 0.001 (one-way ANOVA). H ChIP-PCR assay of the TREM2 promoter. Nrf2 protein–DNA cross-linked samples were obtained from the mPFC of control, susceptible, and resistant mice via coimmunoprecipitation with an anti-Nrf2 antibody. PCR was conducted using TREM2 promoter primers. The data are presented as the mean ± SEM (n = 5). *P < 0.05 (one-way ANOVA). I qPCR assay for the TREM2 promoter and TREM2 in the mPFC. The data are shown as the mean ± SEM (n = 5 or 6). *P < 0.05 and **P < 0.01 (one-way ANOVA). J Western blot assay for Nrf2, TREM2, and arginase 1 protein expression in the mPFC. The data are presented as the mean ± SEM (n = 4 or 6). *P < 0.05 and **P < 0.01 (one-way ANOVA).

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