Fig. 1: Time course of changes in AMPK activity in the NAcsh following intravenous cocaine self-administration (SA).
From: Novel role of AMPK in cocaine reinforcement via regulating CRTC1
A Timeline of the experiment. The rats were trained for saline self-administration, chronic yoked cocaine administration, or cocaine self-administration or maintained in their home cage (HC). They were then decapitated 2 h, 1 day, or 7 days later. B–D, Phosphorylated AMPK (B) and total AMPK (C) protein levels and representative Western blots (D) in the NAcsh 2 h, 1 day, or 7 days after cocaine administration. The data are expressed as a percentage of total AMPK and phosphorylated AMPK in home cage rats (n = 7/group). Post hoc analyses were performed using the Tukey test. ****p < 0.0001, compared with saline self-administration group.
