Fig. 1: Phosphorylation on serine residues of septal HDAC1 is upregulated in response to social fear extinction. | Translational Psychiatry

Fig. 1: Phosphorylation on serine residues of septal HDAC1 is upregulated in response to social fear extinction.

From: HDAC1-mediated regulation of GABA signaling within the lateral septum facilitates long-lasting social fear extinction in male mice

Fig. 1

Schematic representation of the experimental timeline wherein red arrows indicate the time point of sacrifice (A). Mice were fear conditioned (SFC+) or left unconditioned (SFC−) on day 1 of the SFC paradigm. The number of CS-US pairings for groups of SFC+ mice exposed to one social stimulus (1ss) and six social stimuli (6ss) during extinction (B). Total protein was isolated from the septum 90 min either after abridged social fear extinction (SFC−/1ss and SFC+/1ss n = 10/group) or complete extinction (SFC−/6ss and SFC+/6ss; n = 8–9/group; C). Relative protein levels of HDAC1 (D), pHDAC1 (Ser421) (E), and ppHDAC (Ser421, Ser423) (F) were measured by western blot. Representative western blot images for HDAC1, pHDAC1, and ppHDAC1 (G). Data represent mean CS-US pairings ± SEM (B), mean investigation time ± SEM (C), or fold change + SEM (D–F). *P < 0.05 SFC−/6ss vs. SFC+/6ss and #P < 0.05 SFC−/1ss vs. SFC+/1ss (C) or between indicated groups (E, F).

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