Fig. 1: Effects of CSDS on p11 expression in DRN.
From: Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors
A Schematic paradigm of CSDS and experimental timeline for social interaction test (SIT), open field test (OFT), sucrose preference test (SPT) and forced swimming test (FST). B Venn diagram of differentially expressed genes from Ctrl, Sus and Res. C Heatmap showing relative expression levels of 51 differentially expressed genes (DEGs, adjusted p < 0.1 by Deseq2) in the DRN comparing Ctrl with Sus groups. Color scale bar values represent standardized rlog-transformed values across samples. D GO pathway of 51 differentially expressed genes revealed by gene set enrichment analysis (GSEA). GO-BP: gene ontology – biological process, GO-CC: gene ontology – cellular component, GO-MF: gene ontology – molecular function. E Correlation analysis of p11 mRNA expression level and social index (n = 9 p = 0.0027 R = 0.746). F Schematic representation of p11 distribution in DRN. G Immunofluorescence staining of p11, Ctrl-left, Sus-right, scale bar 250 μM. H Comparison of fluorescence intensity of p11 expression in DRN of Sus and Ctrl (Ctrl n = 5, Sus n = 5, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0024 t = 4.366). In all panels, *p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05.
