Fig. 2: Knockdown of p11 in DRN induced depression-like behaviors.
From: Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors
A Experimental paradigm for AAV-hSyn-sh-eGFP and AAV-hSyn-sh-p11 injection (left) and representative picture of injection site verification (middle) and the experimental timeline (right) for social interaction test (SIT), open field test (OFT), sucrose preference test (SPT) and forced swimming test (FST). B Social index in social interaction test of sh-p11 and sh-eGFP group. (sh-eGFP n = 8, sh-p11 n = 12, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.3574 t = 0.9445). C Time spent in the interaction zone in social interaction test of sh-p11 and sh-eGFP group when a target mice (Tg) or no target mice (NTg) in the social zone. (sh-eGFP NTg n = 8, sh-eGFP Tg n = 8, sh-p11 NTg n = 12 sh-p11 Tg n = 12, bars represent mean ± s.e.m. sh-eGFP Tg vs. sh-p11 Tg, Two-tailed unpaired Students t test, p = 0.0319 t = 2.326). D Total distance in the open field test of sh-p11 and sh-eGFP group. (sh-eGFP n = 8, sh-p11 n = 12, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0972 t = 1.762). E Center time in the open field test of sh-p11 and sh-eGFP group. (sh-eGFP n = 8, sh-p11 n = 12, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.1587 t = 1.478). F Center distance in the open field test of sh-p11 and sh-eGFP group. (sh-eGFP n = 8, sh-p11 n = 12, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.1866 t = 1.380). G Percentage of sucrose preference in the sucrose preference test of Ctrl/Res/Sus mice. of sh-p11 and sh-eGFP group. (sh-eGFP n = 7, sh-p11 n = 11, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0409 t = 2.223). H Immobility time in in forced swimming test of sh-p11 and sh-eGFP group. (sh-eGFP n = 8, sh-p11 n = 12, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0296 t = 2.362).
