Fig. 4: p11 overexpression in 5-HTDRN ameliorates stress-induced depression-like behavior.
From: Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors
A Experimental paradigm for AAV-DIO-mCherry and AAV-DIO-p11 injection (left) and experimental timeline for CSDS modeling (middle) and social interaction test (SIT), open field test (OFT), sucrose preference test (SPT) and forced swimming test (FST). B representative picture of injection site verification. C Time spent in the interaction zone in social interaction test of OE-mCherry and OE-p11 group when a target mice (Tg) or no target mice (NTg) in the social zone. (n = 8, bars represent mean ± s.e.m. Ordinary one-way ANOVA followed by Tukey’s post-hoc test, Tg: Ctrl-OE-mCherry vs. CSDS + OE-mCherry, adjusted p = 0.036, CSDS + OE-mCherry vs. CSDS + OE-p11, adjusted p = 0.0463). D Social index in social interaction test of Ctrl+OE-mCherry/ Ctrl+OE-p11/CSDS + OE-mCherry/CSDS + OE-p11 group. (n = 8, bars represent mean ± s.e.m. two-way ANOVA followed by Tukey’s post-hoc test, Ctrl+OE-mCherry vs CSDS + OE-mCherry, adjusted p = 0.3742, CSDS + OE-mCherry vs CSDS + OE-p11, adjusted p = 0.0014). E Total distance in the open field test of Ctrl+OE-mCherry/Ctrl+OE-p11/CSDS + OE-mCherry/CSDS + OE-p11 group. (n = 8, bars represent mean ± s.e.m. two-way ANOVA followed by Tukey’s post-hoc test, Ctrl+OE-mCherry vs CSDS + OE-mCherry, adjusted p = 0.2969, CSDS + OE-mCherry vs CSDS + OE-p11, adjusted p = 0.5315). F Center distance in the open field test of Ctrl+OE-mCherry/Ctrl+OE-p11/CSDS + OE-mCherry/CSDS + OE-p11 group. (n = 8, bars represent mean ± s.e.m. Ordinary one-way ANOVA by Tukey’s post-hoc test, Ctrl+OE-mCherry vs CSDS + OE-mCherry, adjusted p = 0.9630, CSDS + OE-mCherry vs CSDS + OE-p11, adjusted p = 0.008). G Center time in the open field test of Ctrl+OE-mCherry/Ctrl+OE-p11/CSDS + OE-mCherry/CSDS + OE-p11 group. (n = 8, bars represent mean ± s.e.m. two-way ANOVA followed by Tukey’s post-hoc test, Ctrl+OE-mCherry vs CSDS + OE-mCherry, adjusted p = 0.0771, CSDS + OE-mCherry vs CSDS + OE-p11, adjusted p = 0.8643). H Percentage of sucrose preference in the sucrose preference test of Ctrl+OE-mCherry/ Ctrl+OE-p11/CSDS + OE-mCherry/CSDS + OE-p11 group. (n = 8, bars represent mean ± s.e.m. two-way ANOVA followed by Tukey’s post-hoc test, Ctrl+OE-mCherry vs CSDS + OE-mCherry, adjusted p = 0.0005, CSDS + OE-mCherry vs CSDS + OE-p11, adjusted p = 0.0110). I Immobility time in the forced swimming test of Ctrl+OE-mCherry/Ctrl+OE-p11/CSDS + OE-mCherry/CSDS + OE-p11 group. (n = 8, bars represent mean ± s.e.m. two-way ANOVA followed by Tukey’s post-hoc test, Ctrl+OE-mCherry vs CSDS + OE-mCherry, adjusted p = 0.0072, CSDS + OE-mCherry vs CSDS + OE-p11, adjusted p = 0.9504).
