Fig. 6: p11 involved in the expression of GluN2A on cell membrane.
From: Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors
GluN2A (up) and GluN2B (down) protein level were assessed in the membranc (A-left) and cytoplasm (B-left) of ctrl mice and CSDS mice via Western blot. Comparison of grayscale value of GluN2A (up) and GluN2B (down) protein immunoblotting in the membranc (A-right) (GluN2A Ctrl n sample = 3, n mice = 9, CSDS n sample = 3, n mice = 9, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0359 t = 3.110). (GluN2B Ctrl n sample = 3, n mice = 9, CSDS n sample = 3, n mice = 9, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0894 t = 2.233), Comparison of grayscale value of GluN2A (up) and GluN2B (down) protein immunoblotting in the cytoplasm (B-right) (GluN2A Ctrl n sample = 3, n mice = 9, CSDS n sample = 3, n mice = 9, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0021 t = 7.100). (GluN2B Ctrl n sample = 3, n mice = 9, CSDS n sample = 3, n mice = 9, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.2589 t = 1.315). C left: representative graph of immunofluorescence staining of p11(green), middle: representative graph of immunofluorescence staining of GluN2A (red)/dapi(blue), right: co-localization of p11 (green) with GluN2A (red)/dapi(blue). Ctrl-up, CSDS-down. scale bar 5 μM. D Co-immunoprecipitation (Co-IP) was performed to verify protein interaction between p11 and GluN2B (up)/ GluN2A (down). E Pearson correlation coefficient of p11 and GluN2A of Ctrl mice and CSDS mice(Ctrl n = 4, CSDS n = 5, bars represent mean ± s.e.m. Two-tailed unpaired Students t test, p = 0.0404 t = 2.510).
