Fig. 2: Ank3-1b knockout mice showed disturbed sleep patterns and increased slow gamma during sleep, with stronger effects observed during REM sleep than NREM.

A Plots of time spent in NREM (left) and frequency of NREM bouts (right) per hour in which each point represents an hour of measurement. Ank3-1b^KO/KO mice have increased numbers of NREM bouts compared to WT mice (*p = 0.004). B Individual plots of average power spectra during NREM sleep in which each plot represents power measured from an individual mouse with bootstrapped 95% confidence intervals showing variability in power between bouts. C Plots of estimated gamma power (25-45 Hz) in which each point is a gamma power measurement during a NREM bout. Ank3-1b mice have increased slow gamma power compared to WT during NREM sleep (Ank3-1b^KO/KO vs. WT: *p < 0.001; Ank3-1b^KO/+ vs. WT: *p = 0.001; Ank3-1b^KO/KO vs. Ank3-1b^KO/+: *p < 0.001). D Plots of time spent in REM (left) and frequency of REM bouts (right) per hour in which each point represents an hour of measurement. Ank3-1b^KO/+ and Ank3-1b^KO/KO mice have decreased hourly REM sleep duration compared to WT (*p = 0.002 and *p = 0.03, respectively), and Ank3-1b^KO/KO and Ank3-1b^KO/+ mice have decreased REM bouts per hour compared to WT (*p = 0.002 and *p < 0.001, respectively). E Individual plots of average power spectra during REM sleep in which each plot represents an individual mouse with bootstrapped 95% confidence intervals showing variability in power between bouts. F Plots of estimated gamma power (25–45 Hz) in which each point is a gamma power measurement during a REM bout. Ank3-1b mice have increased slow gamma compared to WT during REM sleep (Ank3-1b^KO/KO vs. WT: *p < 0.001; Ank3-1b^KO/+ vs. WT: *p = 0.02; Ank3-1b^KO/KO vs. Ank3-1b^KO/+: *p < 0.001).