Fig. 3: UBLCP1 protein expression pattern in mouse brain and subcellular localization in different cell types in the brain.
From: A novel autism-associated UBLCP1 mutation impacts proteasome regulation/activity
a UBLCP1 is expressed in wild-type post-natal day 4 mouse brain. Sample images from the cortex, hippocampus, and cerebellum are shown. Brain sections were immunolabeled for UBLCP1 (green). Nuclear DNA was labeled with Hoechst (blue). b Representative images of different coronal brain sections of a wild-type adult mouse (28 weeks old) with multiple regions shown. UBLCP1 is expressed in the cortex, striatum, thalamus, hippocampus, and cerebellum. c UBLCP1 localization in neurons is depicted in the left panels with representative images from neurons in the diencephalon, Purkinje cells in the cerebellum, and dopaminergic neurons in the ventral tegmental area. Brain sections were immunolabeled for UBLCP1 (red), and neuronal markers MAP2 (green, top panel), calbindin (green, middle panel), or TH (green, lower panel). UBLCP1 expression in the glial population is depicted in the panels on the right. UBLCP1+ cells (red) are co-labeled with markers of astrocytes (GFAP, green, top panel), microglia (CD11b, green, middle panel), and oligodendrocytes (Olig2, green, lower panel). Nuclear DNA was labeled with Hoechst (blue in all). d UBLCP1 is identified in the nucleolus of neurons, astrocytes, and microglia, and also in the nucleoplasm in neurons. e Nuclear UBLCP1 (in red) co-localizes with the nucleolar marker fibrillarin (in green). All nuclei in the figure were counterstained blue with Hoechst. b Scale bar: 500 µm. a, c–e Scale bar: 10 µm.
