Fig. 4: Pharmacological block of α7nAchRs prevents the effect of HNK on SV recycling leaving the effect of KET unaffected.

A Representative image of Syt1 Ab-uptake IF (magenta) driven by KCl-induced depolarization in DIV21 primary cortical neurons that were treated with vehicle, KET or HNK in the presence or absence of the selective antagonist of α7nAChR bungarotoxin (Bgtx, 50 nM). Immunostaining for VGLUT1 (green) was used to visualize excitatory synapses. B Quantification of experiment in (A). Significance was assessed using two-way ANOVA with Holm-Šídák’s multiple comparison test and is denoted as ****p < 0.0001. Numbers in brackets indicate the number of coverslips analyzed from two to three culture preparations. Scalebar is 5 µM.