Fig. 5: Pharmacological block of α7nAchRs prevents the effect of HNK treatment on SV release competence. | Translational Psychiatry

Fig. 5: Pharmacological block of α7nAchRs prevents the effect of HNK treatment on SV release competence.

From: Hydroxynorketamine, but not ketamine, acts via α7 nicotinic acetylcholine receptor to control presynaptic function and gene expression

Fig. 5

Representative color gradient images of SypHy fluorescence at baseline, upon stimulation with 40 or 900 AP, and upon NH4Cl application in DIV18-21 cortical neurons treated with vehicle and HNK (A) or KET (E) with or without pretreatment with Bgtx. B, F Average traces from experiment in A or E are plotted following min to max feature normalization. Quantification of the RRP (C, G) and TRP (D, H) fractions from the experiment in (A) and (E), respectively. Significance was assessed by two-way ANOVA with Holm-Šídák’s multiple comparison test and marked ***p < 0.001; **p < 0.01; *p ≤ 0.05. Number of recordings each done on an independent coverslip derived from two to three independent culture preparations are indicated in brackets. Scale bar is 5 µm.

Back to article page