Fig. 4: Tsc2+/− mice showed abnormal myelin sheath and SLCP treatment induced MBP expression. | Translational Psychiatry

Fig. 4: Tsc2+/− mice showed abnormal myelin sheath and SLCP treatment induced MBP expression.

From: Amelioration of the brain structural connectivity is accompanied with changes of gut microbiota in a tuberous sclerosis complex mouse model

Fig. 4

a Schematic illustration of the white matter structure, anterior forceps, which was analyzed using TEM; and the gray matter structures, prefrontal cortical regions (PrL and IL), which were analyzed by MBP and PLP fluorescent immunostaining (adapted from Allen brain atlas). b Electron micrographs of axons in the body of anterior forceps for the three groups. Upper panel. The intact myelin sheath in the WT mice. Middle panel. Abnormal myelin sheath in the Tsc2+/− mice. The red arrowhead indicates the space between the axon-myelin interface. The white open arrow indicates myelin sheath detachment. Lower panel. Myelin sheath with mild myelin splits in the Tsc2+/−/SLCP mice as indicated by the blue arrows. c Upper panel. The myelin thickness was plotted as the function of axon diameter. Lower panel. The g-ratio was plotted as the function of axon diameter. d The quantitative bar graph shows the number of axons with the myelin breakdown. e Quantitative analysis of myelinated axons with the diameter ranging from <0.4 to >1.6 μm as indicated. WT (N = 5), Tsc2+/− (N = 5), and Tsc2+/−/SLCP (N = 4) mice were assessed. f A representative fluorescence immunostaining image from each group is shown for visualizing the immunoreactivity of MBP staining. g The quantitative bar graph shows the MBP-positive staining area (%) for the three groups. h A representative fluorescence immunostaining image from each group is shown for visualizing the immunoreactivity of PLP staining. i The quantitative bar graph shows the PLP-positive staining area (%) for the three groups. WT (N = 3), Tsc2+/− (N = 3), and Tsc2+/−/SLCP (N = 3) mice were assessed. Abbreviations: TEM: transmission electron microscopy; MBP: myelin basic protein; PLP: myelin proteolipid protein; fa: anterior forceps; PrL: prelimbic cortex; IL: infralimbic cortex; ACC: anterior cingulate cortex; M1, M2: motor cortex 1 or 2, respectively. Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant, One-way ANOVA test, post hoc Tukey’s test.

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