Fig. 5: LEfSe gut microbiota analysis.
a Histograms of the LDA scores of gut microbacterial taxa that are differentially abundant among the three groups. Taxa with LDA scores greater than 2 are listed according to the taxonomy as indicated. b A cladogram generated from LEfSe analysis depicts a taxonomic representation of statistical differences among the three groups. Each ring represents the next taxonomic level (from center to outer ring: phylum to genus). The size of each dot represents the relative abundance of the taxon. The color represents significant differences in the group when compared to other groups: blue: WT mice, red: Tsc2+/− mice, green: Tsc2+/−/SLCP mice. Yellow dots are no significant differences among the three groups. α < 0.05, Kruskal-Wallis test c Upper panel. Beta-diversity graphs of PCoA on weighted or unweighted UniFrac and Bray-Curtis distance. Lower panel. Beta-diversity graphs of NMDS on weighted or unweighted UniFrac and Bray-Curtis distance. d 3D-plot of principle component analysis is shown. e Histograms of the LDA scores of gut microbacterial taxa that are differentially abundant between WT and Tsc2+/− mice. Taxa with LDA scores greater than 2 are listed according to the taxonomy as indicated. f Histogram graphs that show the relative abundances of bacterial phyla, classes, families, and genera as indicated in each group. *p < 0.05, two-tailed Mann–Whitney test. g Upper panel: A bar graph that shows the relative abundance of the family Christensenellaceae in WT, Tsc2+/−, Tsc2+/−/SLCP mice. Lower panel: A bar graph that shows the relative abundance of the genus Christensenellaceae_R_7_group in WT, Tsc2+/−, Tsc2+/−/SLCP mice. WT (N = 9), Tsc2+/− (N = 8), and Tsc2+/−/SLCP (N = 8) mice were assessed. Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001, n.s. not significant, Kruskal Wallis, post hoc Uncorrected Dunn’s test.
