Fig. 1: NAPE-PLD expression and regulation in human PBMCs.
From: Selective alterations of endocannabinoid system genes expression in obsessive compulsive disorder
a Schematic representation of human NAPE-PLD gene. ATG is the translation start site: filled boxes represent the exons’ translated sequence. In the lower part is shown the CpG island with their sequences and the position of the CpG sites under study. In the upper part are shown the location of primers used for mRNA quantification. b NAPE-PLD relative gene expression in human PBMCs from patients diagnosed with OCD and healthy subjects (CTRL). Scattered plots represent individuals’ mRNA abundance calculated by the Delta-Delta Ct (ΔΔCt) method. *p < 0.05 Mann–Whitney test. c % of DNA methylation represented as scattered dot plots for the individual CpG sites under study as well as for the average (Ave) of the 6 CpG sites. Significant differences are indicated (Bonferroni corrected), *p < 0.05. Values are reported in Supplementary Table 7. Correlation analysis between NAPE-PLD relative gene expression and % of DNA methylation. The % of DNA methylation refers to the CpG 3 (d), CpG 4 (e), and the average (Ave) (f) of the 6 CpG sites under study, while the relative gene expression refers to individuals’ 2^-ΔΔCt values, Y and X bars respectively. Data were compared by Spearman’s rank correlation coefficient: Spearman’s r and p value are reported inside the boxes.
