Fig. 2: Gene expression of the excitatory synaptic marker Vamp2 is altered in SUD and MDD.
From: Extracellular matrix abnormalities in the hippocampus of subjects with substance use disorder
A Gene expression of the presynaptic vesicle marker Vamp2 is increased in both SUD and MDD, but not in the comorbid condition. (B–E) Multiplex immunofluorescent imaging of VAMP2 immunoreactive puncta within a PNN and on the surface of a PVB interneuron. B WFA-labeled PNN (green), C PVB interneuron (blue) and VAMP2 synaptic puncta (red) and spatial overlap (yellow) (arrows indicate overlap). D Composite z-projection of WFA, PVB, and VAMP2. E Zoomed inset of 1D, with arrows denoting spatial overlap of VAMP2 with WFA (yellow puncta). F There were no significant changes in transcription of the synaptic marker Syn1 in any of the diagnostic groups. G–J Multiplex immunofluorescent imaging depicting SYN1 labeling within a PNN surrounding a PVB interneuron. G WFA-labeled PNN (green), H PVB neuron (blue) and SYN1 (red), I composite z-projection of all three channels. J Zoomed inset depicts spatial overlap with of SYN1 puncta with WFA labeling (arrows to yellow puncta). All scalebars are 10 µm. Error bars are mean ± SEM.
