Fig. 6: The effects of IRF3 on ER stress in iPSC-derived astrocytes. | Translational Psychiatry

Fig. 6: The effects of IRF3 on ER stress in iPSC-derived astrocytes.

From: Molecular mechanisms involved in alcohol craving, IRF3, and endoplasmic reticulum stress: a multi-omics study

Fig. 6

A We tested IRF3 siRNA knockdown efficiency and found that among the four individual IRF3 siRNA, siIRF3_2 and siIRF3_3 can knock down IRF3 to ~10% of its baseline (F (4, 10) = 120.2, p < 0.0001). We then chose those two siRNA for the subsequent experiments. B Knockdown of IRF3 in iPSC-derived astrocytes resulted in decrease ER stress (n = 6) F (2, 15) = 17.39, p = 0.0001. C ER stress assays were performed in iPSC-derived astrocytes exposed to vehicle, ethanol (EtOH), acamprosate, naltrexone, ethanol+acamprosate, and ethanol+naltrexone, with and without IRF3 Knockdown using siIRF3_2 (n = 6, the full list of comparison made are presented in Supplementary Table 9). D We used another IRF3 siRNA (siIRF3_3) to confirm our findings as shown in (C) (the full list of comparison made are presented in Supplementary Table 10). siNeg scrambled siRNA.

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