Fig. 2: Effects of MT or Z-YVAD-FMK on NLRP3 inflammasome activation and pyroptosis in HAPI cells treated by CORT. | Translational Psychiatry

Fig. 2: Effects of MT or Z-YVAD-FMK on NLRP3 inflammasome activation and pyroptosis in HAPI cells treated by CORT.

From: Melatonin alleviates chronic stress-induced hippocampal microglia pyroptosis and subsequent depression-like behaviors by inhibiting Cathepsin B/NLRP3 signaling pathway in rats

Fig. 2

A The death of HAPI cells was detected by flow cytometry. B The PI-positive HAPI cells rate. C The relative protein expression of NLRP3, ASC, Cleaved caspase-1, and GSDMD-N. D–G Proteins quantitative analysis of NLRP3, ASC, Cleaved caspase-1, and GSDMD-N. H, I The content of IL-1β, and IL-18. J Relative protein expression of Cleaved caspase-1 and GSDMD-N. K, L Proteins quantitative analysis of Cleaved caspase-1 and GSDMD-N. M–O The contents of IL-1β, IL-18, and LDH. All data are presented as the mean ± SD (n = 6). *P < 0.05 and **P < 0.01 versus CON group. ##P < 0.01 versus CORT group.

Back to article page