Fig. 4: Hippocampal neurogenesis and BDNF levels. | Translational Psychiatry

Fig. 4: Hippocampal neurogenesis and BDNF levels.

From: Exercise mitigates a gut microbiota-mediated reduction in adult hippocampal neurogenesis and associated behaviours in rats

Fig. 4

Immunohistochemical measurement of the survival of new neurons (BrdU and NeuN positive cells) in brain sections through (a) the whole (n = 5; two-way ANOVA; main effect of Abx; main effect of Ex) (b) dorsal (n = 5; two-way ANOVA; main effect of Abx; main effect of exercise; a priori comparisons €p < 0.05) and (c) ventral hippocampus (n = 5; two-way ANOVA). d representative images of BrdU/NeuN at X20 magnification (n = 5). e representative images of DCX+ cells at X40 magnification (f) DCX counts following classification based on their dendritic tree morphology and total count of all DCX+ cells (n = 5; two-way ANOVA; main effect of exercise: a priori comparisons €€p < 0.01; €€€p < 0.001 and main effect of Abx; a priori comparisons $p < 0.05; $$p < 0.01). g BDNF in hippocampus (pg/mg tissue) (n = 5; two-way ANOVA; main effect of exercise) (h) BDNF (ng/ml) in plasma (n = 10; two-way ANOVA; main effect of exercise: a priori comparisons €p < 0.05). Data are graphed as means + SEM. See Supplementary Table 7 for details of statistical analysis.

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