Fig. 2: EZH2 knockdown in the CeA prevents AIE-induced anxiety-like behaviors in adulthood. | Translational Psychiatry

Fig. 2: EZH2 knockdown in the CeA prevents AIE-induced anxiety-like behaviors in adulthood.

From: EZH2-dependent epigenetic reprogramming in the central nucleus of amygdala regulates adult anxiety in both sexes after adolescent alcohol exposure

Fig. 2

A Experimental schematic outlining adolescent intermittent ethanol (AIE) and saline (AIS) procedures, progression into adulthood, surgery schedule, and molecular and behavioral analysis. B AIE induces adulthood anxiety-like behavior in male rats that is prevented by knockdown of EZH2 in the central nucleus of amygdala (CeA) in the elevated plus maze (EPM) test (n = 5–6/group). C AIE induces adulthood anxiety-like behavior in female rats that is prevented by the knockdown of EZH2 in the CeA, in the EPM test (n = 6–7/group). D AIE induces adulthood anxiety-like behavior in male rats during the light/dark box (LDB) test that can be prevented by EZH2 knockdown in the CeA (n = 8/group). E AIE induces adulthood anxiety-like behavior in female rats in the LDB test that can be prevented by EZH2 knockdown in the CeA (n = 6–7/group). Values are mean ± SEM. Statistical significance was determined with two-way ANOVA followed by Tukey’s post hoc test (**p < 0.01, ***p < 0.001). The individual value for each rat is represented by a circle dot on the bar diagram. AIS Adolescent intermittent saline, AIE Adolescent intermittent ethanol, PND Postnatal day.

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