Fig. 4: EZH2 knockdown prevents decreases in Arc protein and mRNA levels after AIE in adulthood in both male and female rats. | Translational Psychiatry

Fig. 4: EZH2 knockdown prevents decreases in Arc protein and mRNA levels after AIE in adulthood in both male and female rats.

From: EZH2-dependent epigenetic reprogramming in the central nucleus of amygdala regulates adult anxiety in both sexes after adolescent alcohol exposure

Fig. 4

A Representative photomicrograph of Arc gold immunolabeling images of the central nucleus of amygdala (CeA) of male rats. Scale bar = 40 µm. Inset photograph shows immunogold particles in a single nucleus (marked with circle in each image) at 100x magnification. B Arc protein expression was decreased after AIE in the CeA and MeA. Decrease in Arc protein expression in the CeA was prevented by EZH2 knockdown in the CeA of AIE male rats (n = 5/group). C Arc mRNA levels were decreased in the amygdala after AIE, and this was prevented by EZH2 knockdown in the CeA in male rats (n = 5-8/group). D Representative photomicrographs of gold immunolabeling images of the CeA of female rats (Scale bar = 40 µm). The inset photograph shows immunogold particles in a single nucleus (marked with circle in each image) at 100x magnification. E Arc protein levels were decreased after AIE in the CeA and MeA, and decrease in Arc protein levels in the CeA was prevented by EZH2 knockdown in the CeA of AIE female rats (n = 5/group). F Arc mRNA was decreased in the amygdala after AIE, and this was prevented by EZH2 knockdown in the CeA in female rats (n = 6-7/group). Values are mean ± SEM. Statistical significance was determined with two-way ANOVA followed by Tukey’s post hoc test (*p < 0.05, ***p < 0.001). The individual value for each rat is represented by a circle dot on the bar diagram. AIS Adolescent intermittent saline, AIE Adolescent intermittent ethanol.

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