Fig. 3: Ketamine-induced changes in gene co-expression patterns.

A Gene dendrogram (top) of WCGNA analysis applied to the VOOM normalized expression matrix of 17,218 genes across all timepoints jointly. A total of 34 co-expression modules were identified using the dynamic split method colored (upper bar). Each line on the dendrogram represents a single gene. Color bars indicate the association of each gene with the respective timepoint (negative correlations in blue, positive correlations in red). B Boxplots of the ME values (y-axis) of the six modules showing significant association (Dunnett’s test, p < 0.05) with time at one or more timepoints (x-axis). C, D GO Functional enrichment plots of the purple (M2; left) and yellow (M4; right) modules, which reflect the two modules with the strongest signal for functional enrichment. Bar color indicates GO functional group, and each bar is labeled with the associated enrichment term. Top significant terms are shown for each functional group, with the -log₁₀ of the multiple test corrected p-values (x-axis) shown for each enrichment term (y-axis).