Fig. 3: BD caudate MSN-associated gene module and hub gene network by WGCNA analysis. | Translational Psychiatry

Fig. 3: BD caudate MSN-associated gene module and hub gene network by WGCNA analysis.

From: Exploring PDE5A upregulation in bipolar disorder: insights from single-nucleus RNA sequencing of human basal ganglia

Fig. 3

A Dendrogram showing the gene modules identified in DEGs between human control and BD basal ganglia nuclei using WGCNA. Hierarchical clustering of genes with topological similarity was shown. Upper band indicated the module assignments after applying the dynamic tree cut algorithm. Lower band showed the gene modules after merge with highly correlated eigengenes. B Heatmap indicating the correlation between module eigengenes and cell type identities. C Heatmap showing the correlation between module eigengenes and other traits, such as number of transcripts per cell (nCount_RNA), number of expressed genes per cell (nFeature_RNA), and identities of control, BD, and brain regions. D, E Bar plot showing the eigengene expression of the gene module green and cyan among cell types between control and BD, respectively. F Network of hub genes identified in module green. The circle size is positively correlated with module membership of genes, and thickness of line is positively correlated with intergenic correlation. G Representative immunofluorescent staining images of human caudate sections stained with DARPP32 (green) and PDE5A (red). Scale bar, 100 μm. Zoom-in image scale bar, 20 μm. Cell ratio of DARPP32 + PDE5A+ in DARPP32+ cells between healthy control (HC) and bipolar disorder (BD) was compared (Shapiro-Wilk test, n = 7 pairs of donors, 2–6 fields per donor, *p < 0.05).

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