Fig. 6: PDE5A overexpression in cultured striatal neurons led to increased neuronal excitability and membrane resistance. | Translational Psychiatry

Fig. 6: PDE5A overexpression in cultured striatal neurons led to increased neuronal excitability and membrane resistance.

From: Exploring PDE5A upregulation in bipolar disorder: insights from single-nucleus RNA sequencing of human basal ganglia

Fig. 6

Whole-cell patch clamp recording was performed on primary cultured mouse E16-17 LGE-derived MSNs infected with control or rAAV2/9-hSyn-PDE5A-EGFP virus (A−E). A Representative traces for neuron spikes elicited by current steps. B The number of action potentials in response to step current injections (from 0 to100 pA: F (10, 320) = 1.337, p = 0.2096; from 0 to 70 pA: F (7, 224) = 2.782, p = 0.0309; 50 pA: p = 0.0185, two-way ANOVA followed by Sidak test). C The slope of the spikes per 10 pA (t (32) = 2.156, p = 0.0248, unpaired two-tailed t-test). D, E Comparison of membrane capacitance and membrane resistance between control and PDE5A OE group, respectively (t (32) = 0.3924, p = 0.6974 for (D); t (32) = 2.556, p = 0.0156 for (E), unpaired two-tailed t-test). All values were presented as mean ± SEM, *p < 0.05. n = 17 neurons from 3 batches of primary culture for each group.

Back to article page