Fig. 7: PDE5A overexpression led to reduced motor balance, sensorimotor gating, sociability and triggered mania-like state.

A series of behavioral tests were performed on control and PDE5A OE mice 4 weeks after stereotaxic injections with one week interval between tests. A Comparisons of run and coordination characteristics by catwalk gaiting analysis (Shapiro-Wilk test). B Comparison of time on rotarod by rotarod test (Shapiro-Wilk test). C Comparisons of the distance moved every 5 min, total distance moved, number of entries into the center, time spent in the center, and duration before first entry into the center by open field test (2 way ANOVA Sidak’s multiple comparison test for left first panel, Shapiro-Wilk test for other panels). D Comparisons of interaction time with stranger mouse 1 (S1) and empty cage (E), interaction time with stranger mouse 2 (S2) and S1, sociability (time spent ratio of (S1 - E) to (S1 + E)), and social novelty (time spent ratio of (S2 - S1) to (S2 + S1)) by three-chamber test (2 way ANOVA Sidak’s multiple comparison test for left first and third panels, Shapiro-Wilk test for other panels). E. Comparison of the percentage of sucrose consumption relative to the total consumption of water and sucrose solution within 36 h by sucrose preference test (Mann–Whitney U test). F Comparison of the percentage of correct alternations in the Y-maze task (Shapiro-Wilk test). G Comparison of pre-pulse inhibition level was assessed at pre-pulse intensities of 74, 78, and 82 dB by PPI test (2 way ANOVA Sidak’s multiple comparison test). H Comparison of immobility time during the last 4 min in water by forced swimming test (Shapiro-Wilk test). n = 10 control and 9 PDE5A OE mice for (A), n = 9 control and 9 PDE5A OE mice for (B−H), due to one death of control group in the process. *p < 0.05; **p < 0.01; ***p < 0.001.