Fig. 1: LTP- and LTD-based screening to identify proteins involved in synaptic plasticity. | Translational Psychiatry

Fig. 1: LTP- and LTD-based screening to identify proteins involved in synaptic plasticity.

From: Electrophysiology-based screening identifies neuronal HtrA serine peptidase 2 (HTRA2) as a synaptic plasticity regulator participating in tauopathy

Fig. 1

A Hippocampal slices from WT mice at 8 months of age were stabilized for 20 min. The hippocampal CA3 region was subjected to high-frequency stimulation (HFS) to induce LTP (in green), low-frequency stimulation (LFS) to induce LTD (in red), or no stimulation as controls (in black). The fEPSPs in the hippocampal CA1 region were then recorded for another 60 min. n = 8 slices from 8 mice for each group. B The numbers of upregulated (Up) and downregulated (Down) proteins in CA1 induced by LTP or LTD. GO (C) and KEGG (D) enrichment analysis of the 20 DEPs shared by both the LTP and the LTD processes. Representative images of HTRA2 (red) and DAPI (blue) in CA1 of 8-month-old WT mice after LTP (E) and LTD (F) inductions (n = 4 slices from 4 mice). Scale bars, 100 μm. HTRA2 immunostaining intensity was quantified for comparison. A significant difference was determined using the Mann-Whitney test (E), and an unpaired t-test (F). Data represent means ± SEM. *p < 0.05; **p < 0.01.

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