Fig. 2: Knockdown of HTRA2 impairs synaptic transmission and ATP production in mouse primary neurons. | Translational Psychiatry

Fig. 2: Knockdown of HTRA2 impairs synaptic transmission and ATP production in mouse primary neurons.

From: Electrophysiology-based screening identifies neuronal HtrA serine peptidase 2 (HTRA2) as a synaptic plasticity regulator participating in tauopathy

Fig. 2

Representative sEPSC (A) and sIPSC (B) traces in neurons infected with shNC and shHTRA2 viruses. Histogram plots of amplitudes (C, E) and frequencies (D, F) of sEPSCs (C, D) and sIPSCs (E, F) (n = 12–17 neurons from 3 independent experiments). G Representative Western blotting and quantification comparison of indicated proteins in neurons infected with shNC and shHTRA2 viruses (n = 4 per group, controls were set to one arbitrary unit and indicated by a dashed line). H Comparison of ATP levels in neurons infected with shNC and shHTRA2 viruses (n = 5 per group). A significant difference was determined using the Mann-Whitney test. Data represent means ± SEM. *p < 0.05; **p < 0.01; ns, not significant.

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