Fig. 3: CaMKIIα P212L Ht mice display ASD- and DCD-related behavioral abnormalities in a comprehensive behavior test battery.

A Male CaMKIIα P212L mice show anxiety-like behavior (unpaired Student’s t-test). B Assessment of spontaneous locomotor activity with a beam break test. A series of beam break counts in 5 min bins are shown in the left panels (two-way repeated measures ANOVA test). Total beam breaks among the 2 h are shown in the right panels (unpaired Student’s t-test). C Aberrant social behavior in CaMKIIα P212L mice. An empty cage, “E”, was placed on the left side, and a stranger mouse, “S”, was placed on the right side of the three chambers. Representative heatmap images on the social approach are shown in the left panels. Time spent around the cage is shown in the right panels (two-way ANOVA test, Tukey post hoc test). D Motor coordination was affected in CaMKIIα P212L mice. The time for mice to fall off the rod during the three-day training period on the accelerated rotarod test (4–40 rpm, 300 s) is shown in the left panels (two-way repeated measures ANOVA test). The time for mice to fall off the rod on the fixed speed rotarod test (24 rpm, 120 s) on the fourth day is shown in the right panels (unpaired Student’s t-test). E CaMKIIα P212L mice display an elevated anxiety level in the self-grooming test (unpaired Student’s t-test). F CaMKIIα P212L mice exhibit enhanced fear memory (two-way repeated measures ANOVA, Šídák post hoc test). Contextual fear memory is shown in the middle panels (unpaired Student’s t-test), and cued fear memory is shown in the right panels (unpaired Student’s t-test). Male: WT; n = 12, Ht; n = 12, Female: WT; n = 12, Ht; n = 12. Data represents mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.