Fig. 2: Demyelination induced by prenatal VPA can be restored by Clemastine. | Translational Psychiatry

Fig. 2: Demyelination induced by prenatal VPA can be restored by Clemastine.

From: Prenatal valproic acid on the basis of gestational diabetes also induces autistic behavior and disrupts myelination and oligodendroglial maturation slightly in offspring

Fig. 2

A At PND 14, 28, 42, and 56, myelin specific CNPase in the CC in the control (e–h) and VPA (i–l) groups were exhibited with immunohistochemical staining, based on the sagittal brain disgrams at different postnatal ages (a–d). B The AOD value of immunohistochemical CNPase in the control and VPA groups on different postnatal days (n = 4). C Myelin specific CNPase in the control and VPA groups in the anterior forceps f, k, genu g, l, body h, m, splenium i, n, and posterior forceps j, o of the CC were exhibited with immunohistochemical staining, based on the coronal brain disgrams of different subregions a–e. D The AOD value of immunohistochemical CNPase in the control and VPA groups in the different subregions of the CC (n = 4). E The three-chamber sociability test in the VPA group a–f and VPA+Clemastine group g–l at PND 56 (n = 6). The location preferences m and athletic ability n in the initial stage, the preference for the object over the mouse in the second stages o, p, and the preference for the strange mouse over the familiar mouse in the third stages q, r were analyzed as mentioned in Fig. 1. F Myelin specific CNPase in the gCC in the control a, VPA b, VPA+clemastine c, and clemastine d groups were exhibited with immunohistochemical staining. G The AOD value of immunohistochemical CNPase in the control, VPA, VPA+clemastine, and clemastine groups (n = 6). ***P < 0.001, *P < 0.05, compared with two groups. #P > 0.05, compared with two groups.

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