Table 1 Key resource table.
No. | Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information | Nationality |
---|---|---|---|---|---|---|
1 | Antibody | Anti-CNPase antibody [1H10] | Abcam | ab277621 | Immunohistochemistry, (1:400) | USA |
2 | Antibody | Myelin Basic Protein (D8X4Q) XP® Rabbit mAb | Cell Signaling Technology | 78896 s | Immunohistochemistry, (1:800) | USA |
3 | Antibody | Olig2 (E6G6Q) XP® Rabbit mAb | Cell Signaling Technology | 65915S | Immunofluorescence, (1:200) | USA |
4 | Antibody | CD140a (PDGFRA) Monoclonal Antibody (APA5) | Thermo Fisher Scientific | 12140181 | Immunofluorescence, (1:100) | USA |
5 | Antibody | Anti-APC (Ab-7) Mouse mAb (CC-1) | Millipore | OP80 | Immunofluorescence, (1:50) | USA |
6 | Antibody | Anti-PDGFR alpha antibody [EPR22059-270] | Abcam | ab203491 | Immunofluorescence, (1:100) | USA |
7 | Antibody | Anti-PCNA antibody [PC10] | Abcam | ab29 | Immunofluorescence, (1:100) | USA |
8 | Antibody | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb | Cell Signaling Technology | 4370 T | Immunofluorescence, (1:100) |  |
Immunoblotting, 1:1000 | USA | Â | Â | Â | Â | Â |
9 | Antibody | Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 | Millipore | mab5384-I | Immunofluorescence, (1:200) | USA |
10 | Antibody | HDAC3 (D2O1K) Rabbit mAb | Cell Signaling Technology | 85057S | Immunoblotting, (1:1000) | USA |
11 | Antibody | beta-Actin Monoclonal Antibody (15G5A11/E2) | Introvergen | MA1-140 | Immunoblotting, (1:3000) | USA |
12 | Antibody | beta Tubulin Loading Control Monoclonal Antibody (BT7R) | Introvergen | MA5-16308 | Immunoblotting, (1:3000) | USA |
13 | Antibody | Lamin B1 antibody | GeneTex | GTX103292 | Immunoblotting, (1:1000) | USA |
14 | Antibody | p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb | Cell Signaling Technology | 4695 T | Immunofluorescence, (1:100) Immunoblotting, (1:1000) | USA |
15 | Antibody | HDAC1 (D5C6U) XP® Rabbit mAb | Cell Signaling Technology | 34589 T | Immunoblotting, (1:1000) | USA |
16 | Antibody | HDAC2 Rabbit Monoclonal Antibody | Beyotime | AF1555 | Immunoblotting, (1:1000) | China |
17 | Antibody | HDAC8 Rabbit Monoclonal Antibody | Beyotime | AF2737 | Immunoblotting, (1:1000) | China |
18 | Antibody | MEK1/2 (D1A5) Rabbit mAb | Cell Signaling Technology | 8727 T | Immunoblotting, (1:1000) | USA |
19 | Antibody | mTOR (7C10) Rabbit mAb | Cell Signaling Technology | 2983 T | Immunoblotting, (1:1000) | USA |
20 | Antibody | Phospho-mTOR (Ser2448) (49F9) Rabbit mAb | Cell Signaling Technology | 2976S | Immunoblotting, (1:1000) | USA |
21 | Antibody | Akt (pan) (11E7) Rabbit mAb | Cell Signaling Technology | 4685S | Immunoblotting, (1:1000) | USA |
22 | Antibody | Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb | Cell Signaling Technology | 4060 T | Immunoblotting, (1:1000) | USA |
23 | Antibody | Alexa Fluor 647-labeled Goat Anti-Rabbit IgG | Beyotime | A0468 | Immunofluorescence, (1:500) | China |
24 | Antibody | Alexa FluorTM 488 Goat anti-Rabbit IgG | Introvergen | A11008 | Immunofluorescence, (1:500) | USA |
25 | Antibody | Alexa FluorTM 488 Goat anti-Rat IgG | Introvergen | A11006 | Immunofluorescence, (1:500) | USA |
26 | Antibody | Alexa Fluor Cy3-labeled Goat Anti-Mouse IgG | Beyotime | A0521 | Immunofluorescence, (1:500) | China |
27 | Antibody | Alexa Fluor 488® Goat Anti-Rabbit IgG H&L | Abcam | ab150077 | Immunofluorescence, (1:1000) | USA |
28 | Antibody | HRP-labeled Goat Anti-Rabbit IgG(H+L) | Beyotime | A0208 | Â | China |
29 | Antibody | HRP-labeled Goat Anti-Mouse IgG(H+L) | Beyotime | A0216 | Â | China |
30 | Kit | Cell Counting Kit-8 | Dojindo | CT04-100 | Â | Japan |
31 | Kit | Universal mouse/rabbit polymer method detection system | ZSGB Biotech | PV-6000 | Â | China |
32 | Kit | DAB chromogenic kit | ZSGB Biotech | ZLI-9019 | Â | China |
33 | Kit | Nuclear protein extraction kit | Solarbio | R0050 | Â | China |
34 | Kit | Bicinchoninic acid protein assay kit | Beyotime | P0012 | Â | China |
35 | Kit | BeyoECL star kit | Beyotime | P0018AS | Â | China |
36 | Kit | Luxol fast blue staining kit | Solarbio | G3245 | Â | China |
37 | Cell line | OLN-93 | Yaji Biotechnology | YS1232C | Â | China |
38 | Serum | Fetal Bovine Serum | Lonsera | S711-001S | 10% | Uruguay |
39 | Formula feeds | High-fat diet (45 kcal/day) | Medicience Biotechnology | MD12032 |  | China |
40 | Reagent | DMEM | Procell Life Science & Technology | PM150210 | Â | China |
41 | Reagent | Penicillin-streptomycin Premix | Beyotime | C0222 | 1% | China |
42 | Reagent | Sodium Valproate | Solarbio | IV0010 | Mice, 600 mg/Kg Cells, 5 mM | China |
43 | Reagent | Clemastine | Institute of Food and Drug Control | ALH8 | Mice, 10 mg/Kg | China |
44 | Reagent | DMSO | Solarbio | D8371 | Â | China |
45 | Reagent | (+)-Glucose | BBI Lifescience | A600219 | Cells, 50 mM | China |
46 | Reagent | Gamma-aminobutyric Acid | Sigma | A2129 | Cells, 100 mM | USA |
47 | Reagent | (+)-Bicuculline | Selleck | S7071 | Cells, 100 μM | China |
48 | Reagent | CGP52432 | Selleck | S0303 | Cells, 25 μM | China |
49 | Reagent | U0126 | Selleck | S1102 | Cells, 10 μM | China |
50 | Reagent | Palmitic acid | Selleck | S3794 | Cells, 200 μM | China |
51 | Reagent | Antifade mounting medium with DAPI | Beyotime | P0131 | Â | China |
52 | Reagent | fat-free BSA | Biofroxx Biotech |  | Cells, 200 μM | China |
53 | Reagent | Sodium Pentobarbital | Sigma | P3636 | 1% | USA |
54 | Reagent | Paraformaldehyde Solution | Servicebio | G1101 | 4% | China |
55 | Reagent | Glutaraldehyde Solution | Servicebio | G1124 | 2.50% | China |
56 | Reagent | RIPA lysis buffer | Beyotime | P0013B | Â | China |
57 | Reagent | Phosphatase inhibitor cocktail A | Beyotime | P1082 | Â | China |
58 | Reagent | Protease inhibitor cocktail | Beyotime | P1008 | Â | China |
59 | Reagent | EDTA | Beyotime | P1005 | Â | China |
60 | Reagent | SDS-PAGE sample loading buffer | Beyotime | P0015 | Â | China |
61 | Reagent | Quick antigen retrieval solution for frozen sections | Beyotime | P0090 | Â | China |
62 | Reagent | Goat serum solution | Solarbio | SL038 | 10% | China |
63 | Reagent | Western rapid transfer buffer | Beyotime | P0572 | Â | China |
64 | Reagent | BeyoGelTM plus SDS-PAGE hepes electrophoresis buffer | Beyotime | P0552 | Â | China |
65 | Reagent | Wb antigen blocking and antibody sensitization diluent | Willget Biotech | F01 | Â | China |
66 | Materials | BeyoGelTM plus precast PAGE gel | Beyotime | P0452 | Tris-Gly, 8%, 15 wells | China |
67 | Materials | PVDF membranes | Millipore | ISEQ00010 | Â | USA |
68 | Markers | PageRulerTM Prestained Protein Ladder | Thermo Fisher Scientific | 26616 | Â | USA |
69 | Markers | PageRulerTM Prestained Protein Ladder | Thermo Fisher Scientific | 26619 | Â | USA |