Table 1 Key resource table.

From: Prenatal valproic acid on the basis of gestational diabetes also induces autistic behavior and disrupts myelination and oligodendroglial maturation slightly in offspring

No.

Reagent type (species) or resource

Designation

Source or reference

Identifiers

Additional information

Nationality

1

Antibody

Anti-CNPase antibody [1H10]

Abcam

ab277621

Immunohistochemistry, (1:400)

USA

2

Antibody

Myelin Basic Protein (D8X4Q) XP® Rabbit mAb

Cell Signaling Technology

78896 s

Immunohistochemistry, (1:800)

USA

3

Antibody

Olig2 (E6G6Q) XP® Rabbit mAb

Cell Signaling Technology

65915S

Immunofluorescence, (1:200)

USA

4

Antibody

CD140a (PDGFRA) Monoclonal Antibody (APA5)

Thermo Fisher Scientific

12140181

Immunofluorescence, (1:100)

USA

5

Antibody

Anti-APC (Ab-7) Mouse mAb (CC-1)

Millipore

OP80

Immunofluorescence, (1:50)

USA

6

Antibody

Anti-PDGFR alpha antibody [EPR22059-270]

Abcam

ab203491

Immunofluorescence, (1:100)

USA

7

Antibody

Anti-PCNA antibody [PC10]

Abcam

ab29

Immunofluorescence, (1:100)

USA

8

Antibody

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb

Cell Signaling Technology

4370 T

Immunofluorescence, (1:100)

 

Immunoblotting, 1:1000

USA

     

9

Antibody

Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38

Millipore

mab5384-I

Immunofluorescence, (1:200)

USA

10

Antibody

HDAC3 (D2O1K) Rabbit mAb

Cell Signaling Technology

85057S

Immunoblotting, (1:1000)

USA

11

Antibody

beta-Actin Monoclonal Antibody (15G5A11/E2)

Introvergen

MA1-140

Immunoblotting, (1:3000)

USA

12

Antibody

beta Tubulin Loading Control Monoclonal Antibody (BT7R)

Introvergen

MA5-16308

Immunoblotting, (1:3000)

USA

13

Antibody

Lamin B1 antibody

GeneTex

GTX103292

Immunoblotting, (1:1000)

USA

14

Antibody

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb

Cell Signaling Technology

4695 T

Immunofluorescence, (1:100)

Immunoblotting, (1:1000)

USA

15

Antibody

HDAC1 (D5C6U) XP® Rabbit mAb

Cell Signaling Technology

34589 T

Immunoblotting, (1:1000)

USA

16

Antibody

HDAC2 Rabbit Monoclonal Antibody

Beyotime

AF1555

Immunoblotting, (1:1000)

China

17

Antibody

HDAC8 Rabbit Monoclonal Antibody

Beyotime

AF2737

Immunoblotting, (1:1000)

China

18

Antibody

MEK1/2 (D1A5) Rabbit mAb

Cell Signaling Technology

8727 T

Immunoblotting, (1:1000)

USA

19

Antibody

mTOR (7C10) Rabbit mAb

Cell Signaling Technology

2983 T

Immunoblotting, (1:1000)

USA

20

Antibody

Phospho-mTOR (Ser2448) (49F9) Rabbit mAb

Cell Signaling Technology

2976S

Immunoblotting, (1:1000)

USA

21

Antibody

Akt (pan) (11E7) Rabbit mAb

Cell Signaling Technology

4685S

Immunoblotting, (1:1000)

USA

22

Antibody

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb

Cell Signaling Technology

4060 T

Immunoblotting, (1:1000)

USA

23

Antibody

Alexa Fluor 647-labeled Goat Anti-Rabbit IgG

Beyotime

A0468

Immunofluorescence, (1:500)

China

24

Antibody

Alexa FluorTM 488 Goat anti-Rabbit IgG

Introvergen

A11008

Immunofluorescence, (1:500)

USA

25

Antibody

Alexa FluorTM 488 Goat anti-Rat IgG

Introvergen

A11006

Immunofluorescence, (1:500)

USA

26

Antibody

Alexa Fluor Cy3-labeled Goat Anti-Mouse IgG

Beyotime

A0521

Immunofluorescence, (1:500)

China

27

Antibody

Alexa Fluor 488® Goat Anti-Rabbit IgG H&L

Abcam

ab150077

Immunofluorescence, (1:1000)

USA

28

Antibody

HRP-labeled Goat Anti-Rabbit IgG(H+L)

Beyotime

A0208

 

China

29

Antibody

HRP-labeled Goat Anti-Mouse IgG(H+L)

Beyotime

A0216

 

China

30

Kit

Cell Counting Kit-8

Dojindo

CT04-100

 

Japan

31

Kit

Universal mouse/rabbit polymer method detection system

ZSGB Biotech

PV-6000

 

China

32

Kit

DAB chromogenic kit

ZSGB Biotech

ZLI-9019

 

China

33

Kit

Nuclear protein extraction kit

Solarbio

R0050

 

China

34

Kit

Bicinchoninic acid protein assay kit

Beyotime

P0012

 

China

35

Kit

BeyoECL star kit

Beyotime

P0018AS

 

China

36

Kit

Luxol fast blue staining kit

Solarbio

G3245

 

China

37

Cell line

OLN-93

Yaji Biotechnology

YS1232C

 

China

38

Serum

Fetal Bovine Serum

Lonsera

S711-001S

10%

Uruguay

39

Formula feeds

High-fat diet (45 kcal/day)

Medicience Biotechnology

MD12032

 

China

40

Reagent

DMEM

Procell Life Science & Technology

PM150210

 

China

41

Reagent

Penicillin-streptomycin Premix

Beyotime

C0222

1%

China

42

Reagent

Sodium Valproate

Solarbio

IV0010

Mice, 600 mg/Kg

Cells, 5 mM

China

43

Reagent

Clemastine

Institute of Food and Drug Control

ALH8

Mice, 10 mg/Kg

China

44

Reagent

DMSO

Solarbio

D8371

 

China

45

Reagent

(+)-Glucose

BBI Lifescience

A600219

Cells, 50 mM

China

46

Reagent

Gamma-aminobutyric Acid

Sigma

A2129

Cells, 100 mM

USA

47

Reagent

(+)-Bicuculline

Selleck

S7071

Cells, 100 μM

China

48

Reagent

CGP52432

Selleck

S0303

Cells, 25 μM

China

49

Reagent

U0126

Selleck

S1102

Cells, 10 μM

China

50

Reagent

Palmitic acid

Selleck

S3794

Cells, 200 μM

China

51

Reagent

Antifade mounting medium with DAPI

Beyotime

P0131

 

China

52

Reagent

fat-free BSA

Biofroxx Biotech

 

Cells, 200 μM

China

53

Reagent

Sodium Pentobarbital

Sigma

P3636

1%

USA

54

Reagent

Paraformaldehyde Solution

Servicebio

G1101

4%

China

55

Reagent

Glutaraldehyde Solution

Servicebio

G1124

2.50%

China

56

Reagent

RIPA lysis buffer

Beyotime

P0013B

 

China

57

Reagent

Phosphatase inhibitor cocktail A

Beyotime

P1082

 

China

58

Reagent

Protease inhibitor cocktail

Beyotime

P1008

 

China

59

Reagent

EDTA

Beyotime

P1005

 

China

60

Reagent

SDS-PAGE sample loading buffer

Beyotime

P0015

 

China

61

Reagent

Quick antigen retrieval solution for frozen sections

Beyotime

P0090

 

China

62

Reagent

Goat serum solution

Solarbio

SL038

10%

China

63

Reagent

Western rapid transfer buffer

Beyotime

P0572

 

China

64

Reagent

BeyoGelTM plus SDS-PAGE hepes electrophoresis buffer

Beyotime

P0552

 

China

65

Reagent

Wb antigen blocking and antibody sensitization diluent

Willget Biotech

F01

 

China

66

Materials

BeyoGelTM plus precast PAGE gel

Beyotime

P0452

Tris-Gly, 8%, 15 wells

China

67

Materials

PVDF membranes

Millipore

ISEQ00010

 

USA

68

Markers

PageRulerTM Prestained Protein Ladder

Thermo Fisher Scientific

26616

 

USA

69

Markers

PageRulerTM Prestained Protein Ladder

Thermo Fisher Scientific

26619

 

USA