Fig. 1: MECIII expressed Latrophilin-2 is topographically organized and associates with distinct circuitry. | Translational Psychiatry

Fig. 1: MECIII expressed Latrophilin-2 is topographically organized and associates with distinct circuitry.

From: Entorhinal cortex layer III Adgrl2 expression controls topographical circuit connectivity required for sequence learning

Fig. 1

(A) (Left) Genotype description of mice used for experiment. (Right) representative image of hippocampal circuit with labelled neurons (NeuN) and Adgrl2 protein. (B, C) (Left) Representative images of Adgrl2-mVenus (Control) and Adgrl2-mVenus;pOxr1-Cre mice (KO) and (Right) line scan of fluorescent intensity measurements across MEC layers (B) and CA1 layers (C) (n = 4 control/KO mice pairs - 2 male, 2 female; P60). (D) AAV experimental schematic to label MECIII neurons in adult pOxr1-Cre mice (P60), targeting proximal MEC (pMEC) using CAG-FLEX-tdTomato-AAV5 and distal MEC (dMEC) using CAG-FLEX-GFP-AAV5. (E) Wfs1 IHC labeling for regional boundary delineation of presubiculum (PrS), parasubiculum (PaS), and MEC termination at the rhinal fissure. Yellow arrows indicate landmarks for start and end of the MEC region. (F) Representative image of pMEC and dMEC labeled MECIII neurons as described in (D), and their axonal projections to the hippocampus (indicated by white arrows). (G) Representative image of distal layer III GFP labelled MEC neurons and layer II labeled Wfs1 IHC.

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