Fig. 3: Adgrl2 deletion in MEC layer III neurons does not affect axon projection topography to the hippocampus. | Translational Psychiatry

Fig. 3: Adgrl2 deletion in MEC layer III neurons does not affect axon projection topography to the hippocampus.

From: Entorhinal cortex layer III Adgrl2 expression controls topographical circuit connectivity required for sequence learning

Fig. 3

(A) Experimental design. AAV injections targeting proximal and distal MEC performed in control and MECIII Adgrl2 knockout (KO) animals at P40, imaging at P60. (B) Representative image of tdTomato labelled proximal MEC neurons for control (top) and KO (bottom) mice. (C) Normalized line intensity scan measurements across the proximal to distal MEC layer 3 for control and KO animals (D, E) Similar as for B-C, except for distal targeted MECIII neurons labeled with GFP expressing AAVs. (F) Representative image of control and KO tdTomato labelled axons in the hippocampal SLM. (G) Line intensity scan measurement across the proximal CA1 (pCA1) to distal subiculum (dSUB) axis in the SLM for control and KO animals. (H) Line intensity scan measurement across CA1 layers comparing layer specific targeting of tdTomato axons for control and KO. Plots and summary graphs shown are means +/− SEM (n = 6 control - 4 male, 2 female; n = 6 KO - 2 male, 4 female). (I–K) Similar as F-H, except for distal MECIII neurons axonal projections in the hippocampus visualized by GFP. Plots shown are means +/− SEM (n = 6 control - 4 male, 2 female; n = 6 KO - 3 male, 3 female).

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