Fig. 4: Adgrl2 deletion from MEC layer III neurons alters contralateral MEC input topology. | Translational Psychiatry

Fig. 4: Adgrl2 deletion from MEC layer III neurons alters contralateral MEC input topology.

From: Entorhinal cortex layer III Adgrl2 expression controls topographical circuit connectivity required for sequence learning

Fig. 4

(A) Experimental design. AAV injections targeting proximal and distal MEC performed in control and MECIII Adgrl2 knockout (KO) animals at P40. Projections to the contralateral MEC layer I are imaged at P60 and analyzed. (B) Representative image of tdTomato labelled proximal MEC axons in contralateral MEC for control (top) and KO (bottom) mice. (C) Line intensity scan measurements across the proximal to distal axis in MEC layer 1 comparing axon localization between control and KO. (D) Summary graph of averaged fluorescence levels for control and KO at proximal (distance 15–25%) and distal (distance 75–85%) positions. (E) (Left) Representative image of control and KO contralateral proximal MEC labelled axons in the MEC layer I. (Right) Line intensity scan measurements across the indicated proximal MEC layers. Shown are normalized fluorescent intensity measurements across MEC layers from deep (layer V/VI) to superficial (layer I) comparing layer specific targeting of tdTomato axons for control and KO. Plots and summary graphs shown are means +/− SEM (n = 6 control - 4 male, 2 female; n = 6 KO - 2 male, 4 female). (F–I) Similar as for B-E, except for contralateral distal MEC projections. Plots and summary graphs shown are means +/− SEM (n = 6 control - 4 male, 2 female; n = 6 KO - 3 male, 3 female). Statistical analyses for summary graphs was performed using Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001).

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