Fig. 5: Adgrl2 deletion from MEC layer III neurons alters presubiculum input topology. | Translational Psychiatry

Fig. 5: Adgrl2 deletion from MEC layer III neurons alters presubiculum input topology.

From: Entorhinal cortex layer III Adgrl2 expression controls topographical circuit connectivity required for sequence learning

Fig. 5

(A) AAV experimental schematic targeting PrS using CaMK2a-EYFP-AAV5 to label proximal presubiculum neurons. (B) (Left) Representative image of YFP labelled proximal PrS neurons for control and KO mice. (Right) Line intensity scan measurement across the proximal to distal PrS layer 3 comparing localization of injection for control and KO. (C) Representative image of YFP labelled PrS axons in ipsilateral MEC for control (top) and KO (bottom) mice. (D) Line intensity scan measurement across the proximal to distal MEC layer III comparing axon localization between control and KO. (E) Summary graph of averaged fluorescence levels for control and KO at proximal (distance 15–25%) and distal (distance 75–85%) positions. Statistical analyses were performed using Student’s t-test (**p < 0.01, ***p < 0.001). (F–H) Similar as C-E, except for the contralateral MEC. (I) (Left) Representative image of control and KO PrS labelled axon projections into the ipsilateral MEC layer III. (Right) Line intensity scan measurement across MEC layers comparing layer specific targeting of PrS axons for control and KO. (J) Similar as I, except for contralateral MEC. Plots and graphs shown are means +/− SEM (n = 5 control – 3 male, 2 female; n = 5 KO - 3 male, 2 female).

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