Fig. 5: BA9 EVs are taken up by glial cells, and EVs alter glial cell gene expression. | Translational Psychiatry

Fig. 5: BA9 EVs are taken up by glial cells, and EVs alter glial cell gene expression.

From: Lipidomic and proteomic insights from extracellular vesicles in the postmortem dorsolateral prefrontal cortex reveal substance use disorder-induced brain changes

Fig. 5

A, B Representative 10x images of A human primary astrocytes and B HMC3 microglia incubated with SytoSELECT (green fluorescence)-labeled BA9 EVs (50 μg) in the presence of NucBlue (a live cell stain, 30 µL/mL) and seeded in glass-bottom 96-well plates. Images were taken at 6 and 24 h using an automated Lionheart FX microscope (BioTek). C, D Uptake of BA9 EVs by C astrocytes and D microglia was analyzed by quantifying 3 fields of view with Gen5 software and presented as raw relative florescence units (RFUs). E, F Gene expression of E human primary astrocytes and F HMC3 microglia treated with control or SUD BA9 EVs was assessed by RT‒qPCR. Cells treated with PBS were used as a negative control. For panels C and D, statistical significance was determined by two-way ANOVA. **** p < 0.0001, *** p 0.0002 - 0.0009, ns = nonsignificant. For panels E and F, statistical significance was determined by ordinary one-way ANOVA (Šídák’s multiple comparisons test) and an unpaired t test with Welch’s correction. ****p < 0.0001, ***p 0.0002–0.0009, **p 0.0042, *p 0.0247, ns nonsignificant.

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