Fig. 1: Clustering multiple pooled batches of female and male mice into sustained and phasic responders following auditory aversive conditioning. | Translational Psychiatry

Fig. 1: Clustering multiple pooled batches of female and male mice into sustained and phasic responders following auditory aversive conditioning.

From: Balancing ethics and statistics: machine learning facilitates highly accurate classification of mice according to their trait anxiety with reduced sample sizes

Fig. 1

a Schematic representation of the study. b Average freezing responses of female compared to male mice during memory retrieval (MR) 1. c Histogram of average freezing responses during the last 3.5 min of cued stimulus (CS) presentation (bins 18–24) in MR1 for female and male mice. d Fitted freezing curves for sustained and phasic female responders during CS presentation in MR1. Fitted curves based on group average responses are indicated by thicker lines. R2-values correspond to the goodness-of-fit for the average fitted responses. e Bivariate scatter plots showing clustering of sustained and phasic female responders including 95% confidence ellipses around each cluster. f Freezing responses of sustained and phasic female responders during MR1. Average freezing responses are indicated by thicker lines whereas individual animal freezing curves are shown with lighter colors. g Fitted freezing curves for sustained and phasic male responders during CS presentation in MR1. Fitted curves based on group average responses are indicated by thicker lines. R2-values correspond to the goodness-of-fit for the average fitted responses. h Bivariate scatter plots showing clustering of sustained and phasic male responders including 95% confidence ellipses around each cluster. i Freezing responses of sustained and phasic male responders during MR1. Average freezing responses are indicated by thicker lines whereas individual animal freezing curves are shown with lighter colors. The time bins of cued stimulus (CS) presentation are colored in green (bins 13–17, first 2.5 min of CS presentation) and magenta (bins 18–24, last 3.5 min of CS presentation) in b, d, f, g, and i, corresponding to the phasic and sustained component of the freezing curve as described in Kovlyagina et al. [9] Values are shown as mean ± standard error of the mean in b, f and i. *** p < 0.001, **p < 0.01, *p < 0.05, linear mixed effect models followed by post-hoc comparisons of model means for female vs. male mice in b or sustained vs. phasic responders in f and i. N = 168 females (78 sustained and 90 phasic), n = 224 males (136 phasic and 88 sustained).

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