Fig. 2: Gene expression analysis of inflammatory markers in the liver of male animals exposed to prenatal stress.

The mRNA levels for IL-1β, MIF, CD68, IL-4 and IL-10 were measured in control (CTRL) and prenatally stressed group (PNS) (a) or after the separation in vulnerable (VULN) and resilient (RES) (c). The z-score was calculated based on the mRNA levels of IL-1β, MIF, CD68, IL-4 and IL-10 genes considering whole PNS group (b) or after the separation in vulnerable (VULN) and resilient (RES) (d). Statistical analysis for panels a, b: t-test, *p < 0,05 and **p < 0,01 (n = 14 to 30 per group). Statistical analysis for panels c, d: one-way ANOVA, Tukey’s post hoc, *p < 0,05, **p < 0,01 and ***p < 0,001 (n = 9 to 19 per group). Data are expressed as mean ± SEM and individuals are represented as dots.