Fig. 3: FPI suppresses DMS cholinergic activity. | Translational Psychiatry

Fig. 3: FPI suppresses DMS cholinergic activity.

From: Traumatic brain injury exacerbates alcohol consumption and neuroinflammation with decline in cognition and cholinergic activity

Fig. 3

a Confocal micrographs showing striatal sections from the EtOH+FPI and naïve groups, with CINs in green. Bilateral striatal sections were prepared 5 weeks post-FPI. D, dorsal; M, medial. b FPI significantly decreased the number of CINs per slice in both water and alcohol-exposed groups compared to naïve controls. c FPI reduced the spontaneous firing rates of DMS CINs. Bilateral DMS slices were prepared 1-week post-FPI. d FPI reduced the number of evoked action potentials in DMS CINs. e FPI did not significantly alter the resting membrane potential of DMS CINs. f FPI significantly increased the SAG in DMS CINs. g Confocal micrographs show acetylcholine sensor expression in the striatum of wild-type mice. D, dorsal; L, lateral. h-i FPI reduced the frequency of acetylcholine events in the dorsomedial striatum. n.s. (not significant; e), #p = 0.07, *p < 0.05, **p < 0.01, ***p < 0.001. Two-way RM ANOVA followed by Tukey post-hoc test (d), unpaired t-test (b, c, e, f, i).

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