Fig. 4: Developing and adult TcMAC21 cerebella exhibited disrupted Purkinje neuron subtype antigen Hsp25 expression pattern.

A The sagittal schematics above the data panels indicate the lobules that show parasagittal stripes of Hsp25 (Red), or its absence (Gray); and correspond to the tissue sections shown in panels below. B At P7, Hsp25 immunoreactivity (ir)(Hsp25, gray or red) shows diminished expression in the posterior lobe (dotted rectangle) of euploid cerebella (top, empty arrowheads), while expression remains widespread in TcMAC21 mice (bottom, filled- arrowheads), indicating disrupted patterning. The presence of DAPI counterstaining (blue) indicated the absence Hsp25-ir was not due to artifact. Scale bars: 500 μm on left-panels and 100 μm on the right-panels C The atypical HSP25 distribution persists at P14 in TcMAC21 cerebella (bottom), differing from the typical restricted pattern seen in euploids (top). Note the expanded expression domain (dashed rectangle) compared to the normal restricted pattern. D In adult mice, the coronal schematics indicate Hsp25-positive Purkinje neurons show specific patterning in the nodular zone of control cerebella. E TcMAC21 mice display reduced Hsp25-positive Purkinje neurons (Hsp25, gray or red) in the medial parasagittal stripes, but wider parasagittal stripes were seen in euploid mice. Scale bars: 500 μm and 50 μm (insets). Quantification was obtained from n = 4 mice of each genotype (both sexes included), showing a significantly decrease in both the cell number F and percentage (G) of Hsp25+ Purkinje neurons in adult TcMAC21 mice.